ADVANTAGE MALARIA PAN +Pf CARD is a rapid, visual and sensitive immunoassay for the qualitative diagnosis of infection with P.falciparum and other Plasmodium species (P.falciparum/P.vivax/P.malariae/P.ovale) in human whole blood only.
ADVANTAGE MALARIA PAN +Pf CARD is an immunoassay based on the "Sandwich” principle. The conjugate contains colloidal gold conjugated to Pf specific monoclonal anti- HRP-2 antibody and monoclonal anti-pan specific pLDH antibody. The test uses monoclonal anti-Pf HRP-2 antibody (test line F) & monoclonal anti-pan specific pLDH antibody (test line P) immobilized on a nitrocellulose strip. The test sample is added to the device. On addition of assay buffer, the red blood cells get lysed. If the sample contains P.falciparum or P.vivax/P.malariae/P.ovale, the colloidal gold conjugate complexes the Pf specific HRP-2/ Pan specific pLDH in the lysed sample. This complex migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding immobilized antibody, the complex is trapped forming a purplish pink band which confirms a reactive test result. Absence of a coloured band in the test region indicates a negative test result. To serve as a procedural control an additional line of anti-mouse antibody has been immobilized on the strip as control.